Figure 4.

NOG2 intron growth-defect analysis

(A) Growth in liquid YPG medium of synXIV (strain 56; Table S1) strains integrated with megachunks W, X, and with wild-type NOG2 (strains 57, 59, and 63; Table S1). Lines and error bars represent mean and ±1 standard deviation of A₆₀₀ from biological triplicates.

(B) Integration of megachunk W (strain 57; Table S1) but not chunks W3-W4 (strain 58; Table S1) causes a growth defect in SynXIV.17.

(C) Re-insertion of the NOG2 intron in the synXIV.17c strain restores wild-type fitness (strain 63; Table S1). Spot assays are 10-fold serial dilutions of exponentially growing cultures on YPG medium at 37°C. Images were taken after 5 days (B) and 3 days (C).