REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
NEB 5-alpha Competent E. coli (High Efficiency)	New England Biolabs	C2987H
Chemicals, peptides, and recombinant proteins
Mitotracker Red FM	ThermoFisher	M22425
Β-Estradiol	Merck	E8875
hygromycin B	Merck	10843555001
Deposited data
SynXIV 29 I J1.4 raw genome sequencing data	This study	Biosample: SAMN28591717
SynXIV 29 I J1.8 raw genome sequencing data	This study	Biosample: SAMN28591718
BYe1 raw genome sequencing data	This study	Biosample: SAMN28591711
BYe2 raw genome sequencing data	This study	Biosample: SAMN28591712
BYe3 raw genome sequencing data	This study	Biosample: SAMN28591713
J1.8e1 raw genome sequencing data	This study	Biosample: SAMN28591714
J1.8e2 raw genome sequencing data	This study	Biosample: SAMN28591715
J1.8e3 raw genome sequencing data	This study	Biosample: SAMN28591716
SynXIV.17c NOG2 wt, A2-A3, V4, V1, R1, YNL114W, Chr12, K3, YNL116WL697I, E3 raw genome sequencing data	This study	Biosample: SAMN28591719
Experimental models: Organisms/strains
BY4741	Euroscarf	Strain 1, Table S1
SynXIV 29 I J1.4	This study	Strain 39, Table S1
SynXIV 29 I J1.8	This study	Strain 40, Table S1
BYe1	This study	Strain 41, Table S1
BYe2	This study	Strain 42, Table S1
BYe3	This study	Strain 43, Table S1
J1.8e1	This study	Strain 44, Table S1
J1.8e2	This study	Strain 45, Table S1
J1.8e3	This study	Strain 46, Table S1
SynXIV 7c	This study	Strain 52, Table S1
SynXIV 12c	This study	Strain 53, Table S1
SynXIV.17	This study	Strain 55, Table S1
SynXIV.17.c	This study	Strain 60, Table S1
SynXIV.17.c NOG2 wt	This study	Strain 63, Table S1
SynXIV.17c NOG2 wt, A2-A3, M3	This study	Strain 68, Table S1
SynXIV.17c NOG2 wt, A2-A3, V4, V1, YNL114W	This study	Strain 69, Table S1
SynXIV.17c NOG2 wt, A2-A3, V4, V1, R1, YNL114W, Chr12, K3, YNL116WL697I, E3, R1	This study	Strain 78, Table S1
WWWW	This study	Strain 90, Table S4
WWWS	This study	Strain 91, Table S4
WWSS	This study	Strain 92, Table S4
WSSS	This study	Strain 93, Table S4
Oligonucleotides
ACTCCACTTCAAGTAAGAGTTTG	Integrated DNA Technologies	Mat-A_F
GCACGGAATATGGGACTACTTCG	Integrated DNA Technologies	Mat-alpha_F
AGTCACATCAAGATCGTTTATGG	Integrated DNA Technologies	Mat-locus_R
AGCTTGGTGAGCGCTAGGGAG	Integrated DNA Technologies	his3up-F
GTTCTTACGGAATACCACTTGCC	Integrated DNA Technologies	his3up-R
AACCGGCTTTTCATATAGAATAGAGAAGC	Integrated DNA Technologies	leu2up-F
GAGGTCGACTACGTCGTTAAGG	Integrated DNA Technologies	leu2up-R
Recombinant DNA
pHK-Cre-EBDh	This study	pHK-Cre-EBDh
TAR1-pRS413	This study	TAR1-pRS413
MRPL19-GFP-MRPL19-LoxP-pRS416	This study	MRPL19-GFP-MRPL19-LoxP-pRS416
MRPL19-GFP-MRPL19-Native-pRS416	This study	MRPL19-GFP-MRPL19-Native-pRS416
NOG2wt-GFP-pRS416	This study	NOG2wt-GFP-pRS416
NOG2syn-GFP-pRS416	This study	NOG2syn-GFP-pRS416
Software and algorithms
Image J	National Institutes of Health	github.com/imagej/ImageJ
Prism 9	GraphPad	GraphPad Prism 9.3.1
Geneious Prime	Biomatters Ltd	2022.1.1
Albacore	www.albacorebuild.net	Albacore v2.3.1
Porechop	https://github.com/rrwick/Porechop	Porechop v0.2.3
Canu	https://github.com/marbl/canu	Canu v1.7.1
Nanopolish	https://github.com/jts/nanopolish	Nanopolish 0.10.1
Microsoft Powerpoint	Microsoft Office Professional Plus 2019	Version 1808
Microsoft Excel	Microsoft Office Professional Plus 2019	Version 1808
Guppy	Oxford nanopore Technologies	v4.2.3