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. 2023 Nov 10;36:11947. doi: 10.3389/ti.2023.11947

TABLE 1.

Summary of findings of studies investigating stem cell therapy delivery during ex situ dynamic organ preservation identified after systematic search of the literature (details in Supplementary Material). Results from preliminary studies investigating extracellular vesicle therapy during machine perfusion of transplantable organs are also summarized.

Studies investigating mesenchymal stromal cell delivery during dynamic organ preservation
Study Subject Organ Organ transplant Type and duration MP MSC type MSC dose MSC paracrine activity during MP Device barrier MSC migration from vascular space MSC engraftment a MSC Viability MSC therapeutic effect Effect without engraftment
[36] Human Lungs (discarded) No Normothermic, 4 h Human 5*106 NA NA NA NA NA ↑ alveolar fluid clearance NA
BM-MSC
[8] Human Lungs (discarded) No Normothermic, 4 h MAPc 107 NA NA NA NA NA ↓ BAL cellularity & histological inflammation NA
[9] Pig Lungs No Normothermic, max 12 h Human 50*106 NA Yes, MSC trapped in filters Yes, some cells in the lumen at histology Yes,<10 cells/HPF NA ↓ IL-8 perfusate concentration NA
UC-MSC 150*106
300*106
[10] Rat Kidneys No Hypothermic, 4 h Rat 3*106 NA NA Yes Yes,<10 cells/HPF NA ↓ severity histological damage NA
BM-MSC
[37] Pig Lungs No Normothermic, 6 h MAPc 150*106 No NA No No NA No significant therapeutic effect NA
[34] Mouse Lungs No Normothermic, 1 h Human 3*106 NA NA NA NA NA ↑ compliance NA
UC-MSC ↓ inflammation, neutrophil infiltration & oedema
[38] Rat Liver No Normothermic, 2 h Swine 0.2*106 NA NA NA NA NA No significant therapeutic effect NA
AD-MSC 106
[11] Human Kidneys (discarded) No Sub-normothermic, 24 h Not specified 25*106 Yes No No, 95% MSC still circulating at the end of MP No NA ↑ renal cell proliferation & tissue regeneration Yes
50*106
75*106
1*108
2*108
[12] Pig Kidneys No Normothermic, 7 h Human 1*105 NA Yes, Inhomogeneous distribution in well perfused kidneys No No Disintegrated MSC in colonized glomeruli Study investigating feasibility and biodistribution NA
AD-MSC & 1*106
BM-MSC 1*107
[13] Pig Lungs Yes, f-up 4 h Normothermic, 12 h Human 50*106/Kg Yes NA Yes, Unspecified proportion of MSC remained in the lumen Yes, alveolar interstitium Yes, indirect evidence based on production of human cytokines During MP: ↓ apoptosis & perfusate concentration of IL-18 and IFNγ, ↓ peak airways pressure NA
UC-MSC Post-transplant: ↓ oedema & severity histological injury, f-up limited to 4 h
[14] Human Kidneys (discarded) No Normothermic, 7 h MAPc 50*106 Yes No Yes, Unspecified proportion of MSC kept circulating at the end of MP Yes, glomeruli in the cortex, peritubular space in the medulla 21% of circulating MSC were viable ↑ urinary output & medullar flow NA
↓ urinary concentration NGAL & perfusate concentration IL-1β
↑ perfusate concentration IL-10
[15] Human Liver No Normothermic, 6 h MAPc 50*106 Yes Yes, MSC infused via left hepatic vessels did not reach right segments Yes, only if infused via the hepatic artery Yes, only if infused via the hepatic artery NA ↓ perfusate concentration pro-inflammatory cytokines Yes
↑ perfusate concentration anti-inflammatory cytokines
[16], [39] Rat Liver No Normothermic, 8 h Rat 1–3*107 NA NA No No NA ↓ perfusate AST/ALT and severity histological damage Yes
BM-MSC ↓ mitochondrial injury
[17] Pig Liver No Hypothermic for MSC delivery, 30 min Human 5*106 Yes Yes, inhomogeneous distribution in well perfused livers Yes Yes Yes, indirect evidence based on production of human cytokines Study investigating feasibility and biodistribution NA
Normothermic for functional assessment, 4 h BM-MSC 1*107
[12] Pig Kidneys No Normothermic, 7 h Human 1*107 Yes NA NA NA No significant therapeutic effect NA
AD-MSC & BM-MSC
[18] b Pig Kidneys Yes, f-up 14 days Normothermic, 4 h Human 1*107 NA NA NA Yes, Y human chromosome detected in parenchyma but circa 20-fold ↓ 14 days post-transplant NA No safety concern during perfusion, No significant post-transplant therapeutic effect NA
AD-MSC
[19] Rat Liver Yes, f-up 14 days Normothermic, 4 h Rat 1*107 NA NA No No NA ↓ post-transplant AST/ALT release & acute cellular rejection Yes
BM-MSC c
[20] Rat Liver No Normothermic, 6 h Rat 1*107 NA NA No No NA ↓ severity of ferroptosis & perfusate AST/ALT concentration Yes
BM-MSC 3*107
[21] Rat Liver Yes, f-up 14 days Normothermic, 4 h Rat 1–3*107 NA NA NA Unspecified location in the hepatic tissue NA During MP: ↑ proliferation cholangiocyte extrahepatic bile duct and preservation of epithelial lining NA
BM-MSC c Post-transplant: ↓ AST/ALT/GGT/bili 7 days post-transplant; ↑ proliferation & ↓ apoptosis peribiliary glands
Studies investigating extracellular vesicles delivery during dynamic organ preservation
Study Subject Organ Organ transplant Type and duration MP Source of EV EV dose EV uptake confirmed EV therapeutic effect Compared to MSC
[22] Human Lungs (discarded) No Normothermic, 6 h Human BM-MSC 100–200 μL In vitro only (human alveolar epithelial type 2 cell line) ↑ alveolar fluid clearance & ↓ oedema and weight gain, ↑ compliance No
[10] Rat Kidneys No Hypothermic, 4 h Rat BM-MSC Concentration not reported, EV released by 3*106 cells NA ↓ perfusate LDH and MDA, ↑ glucose metabolism,↓ severity histological damage Yes, magnitude of effects of EV > MSC
[34] Mouse Lungs No Normothermic, 1 h Human UC-MSC Concentration not reported, EV released by 3*106 cells NA ↑ compliance, ↓ inflammation, neutrophil infiltration & oedema Yes, magnitude of effects of EV = MSC
[23] Rat Liver No Normothermic, 4 h Human liver stem-like cells 5*108 EV/g of liver Yes, intracellular localization in hepatocytes ↓ perfusate AST & severity histological damage No
[24] Rat Lungs No Normothermic, 3 h Human BM-MSC 24.56 ± 5.53 *1010 EV/mL, 5 mL were administered Yes, intracellular localization in alveolar cells ↓ total vascular resistance, ↑ glucose metabolism and tissue content of ATP No
[25] Rat Liver No Normothermic, 6 h Human liver stem-like cells 5*108 EV/g of liver Yes, intracellular localization in hepatocytes ↓ perfusate AST/ALT & ↑ bile excretion, ↓ necrosis & ↑ hepatocellular proliferation No
25*108 EV/g of liver
[26] Human Kidneys (discarded) No Hypothermic, 4 h Human BM-MSC 28.5*10^9 NA ↓ apoptosis & ↑ tubular cells proliferation, ↓ mitochondrial injury No
a

Refers to the visualization of MSC between parenchymal cells (outside of the vascular lining) at histology. When available, the estimated cellular concentration is reported.

b

In this study, porcine kidneys underwent 14 h preservation with hypothermic oxygenated MP, followed by 4 h of normothermic MP with or without MSC infusion.

c

In these studies, MSC were modified to overexpress the enzyme heme oxygenase 1.

Abbreviations: AD-MSC, adipose-derived mesenchymal stem cells; ALT, alanine transaminase; AST, aspartate transaminase; ATP, adenosine triphosphate; BAL, bronchoalveolar lavage; BM-MSC, bone marrow-derived mesenchymal stem cells; EV, extracellular vesicles; HPF, high-power field; IFNγ, interferone gamma; IL-1β, interleukin 1 beta; IL-8, interleukin 8; IL-10, interleukin 10; IL-18, interleukin 18; LDH, lactate dehydrogenase; MAPc, multipotent adult progenitor cells; MDA, malondialdehyde; MP, machine perfusion; NGAL, neutrophil gelatinase-associated lipocalin; UC-MSC, umbilical cord-derived mesenchymal stem cells.

NA, or not applicable, is assigned when a manuscript reported insufficient details for accurate evaluation.