Fig. 1. Enhanced expression of ID1 in TAMs correlates with poor clinical outcome for CRC patients.

a Schematic diagram for screening the upregulated oncogenic transcription factors (Log₂FC > 1.5) in peritoneal macrophages (PMs) stimulated with CT26 cell-derived conditional medium (CM) compared to the untreated PMs (left) and the screened 10 upregulated genes (right) by using GEO dataset of GSE80065. b, c Immunoblots of indicated proteins in RAW264.7 cells treated with CT26-derived CM (b) or MC38-derived CM (c), n = 3 biologically independent samples. d–f Representative multiple immunohistochemistry (mIHC) images of Id1 and F4/80 in tumor tissues of AOM/DSS-induced CRC model (d), Apc^Min spontaneous CRC model (e) or in the normal colon tissues of C57BL/6J mouse (f). Scale bar, 25 μm, n = 3 biologically independent samples. g, h The strategy of isolating TAMs from orthotopic MC38 tumor-bearing mice or PMs from normal C57BL/6 J mice (g). The mRNA (h, left) and protein abundance (h, right) of Id1 in TAMs and PMs were detected, n = 3 mice per group, Student’s t-test. i, j Representative mIHC staining of ID1 and CD68 in tumor and adjacent normal tissues from CRC patients (i), and the related statistical data of ID1 expression within CD68⁺ cells (j), Mann–Whitney U test. Scale bar, 25 μm. k Kaplan–Meier plot of overall survival of CRC patients stratified by ID1 expression level within CD68⁺ cells, Log-rank test. Unless specified otherwise, the data are presented as means ± SEM. g is created with BioRender.com. Source data are provided as a Source Data file.