Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 23;14:7661. doi: 10.1038/s41467-023-43548-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Screening for ID1-interacting transcription factors responsible for CCL4 and SerpinB2 transcription. b Co-immunoprecipitation (Co-IP) of ID1 with STAT1 in HEK 293T cells transfected with STAT1-DDK and ID1-GFP expressing plasmids. n = 1 biologically independent sample. c Immunofluorescent staining of Id1 and Stat1 in TAM-like RAW264.7 cells. Scale bar, 10 μm, n = 3 biologically independent samples. d Proximity ligation assay (PLA) for defining the interaction between Id1 and Stat1 in TAM-like RAW264.7 cells. Scale bar, 10 μm, n = 10 biologically independent samples. e Immunofluorescence staining of ID1, STAT1 and CD68 in tumor tissue from CRC patients. Scale bar, 20 μm, n = 3 biologically independent samples. f Analysis of the luciferase activity of truncated promoter sequences of Ccl4 and Serpinb2 in Ctrl or Id1^OE RAW264.7 cells, n = 3 biologically independent samples, Student’s t-test. g Effects of Id1 on Stat1 and p-Stat1 subcellular localization under IFN-γ stimulation in RAW264.7 cells, n = 3 biologically independent samples. h The effects of ID1 on the interaction between STAT1 and CRM1, n = 3 biologically independent samples. i Representative images of PLA and the statistical data showing the protein interaction between STAT1 and CRM1 with ID1 ectopic expression or not, n = 20 biologically independent cells. Scale bar, 5 μm. j Representative images of PLA showing the effects of ID1 on the dimerization and subcellular localization of STAT1 under the treatment of leptomycin B (100 nM), Scale bar, 5 μm, n = 3 biologically independent samples. Source data are provided as a Source Data file.