Figure 1.

Establishing chromosome-scale design-build-test-learn cycles (chrDBTLs) with synXV

We established chrDBTLs by developing a rational chromosome reconfiguration method, CRISPR-Cas9-mediated mitotic recombination with endoreduplication (CRIMiRE). CRIMiRE was applied to accelerate the construction of synXV and to generate a rationally designed semisynthetic synXV library. The ease of generating chromosome libraries by CRIMiRE provided systematic approaches to advance chrDBTL and facilitate the identification of causal variants. We also attested that the redesigned synXV functions as a “build-to-learn” platform and serves as a parallel living system for probing and answering fundamental biological questions.