Table 2.
Transition temperatures of thermal unfolding of VcCBP in the presence of GlcNAc and (GlcNAc)n (n = 2, 3, 4, 5, or 6).
| Tm (°C) | ΔTm (°C) | |
|---|---|---|
| VcCBP alone | 43.2 ± 1.2 | |
| + GlcNAc | 46.0 ± 1.0 | 2.8 |
| + (GlcNAc)2 | 54.4 ± 0.1 | 11.2 |
| + (GlcNAc)3 | 54.8 ± 0.3 | 11.6 |
| + (GlcNAc)4 | 53.8 ± 0.0 | 10.6 |
| + (GlcNAc)5 | 49.6 ± 0.3 | 6.3 |
| + (GlcNAc)6 | 49.6 ± 0.2 | 6.3 |
The unfolding experiments were conducted in 20 mM Tris–HCl buffer pH 8.0, monitoring the CD value at 222 nm using a Jasco J-720 spectropolarimeter (0.1 cm of cell length). Final concentrations of the protein and (GlcNAc)n were 8 µM and 8 mM, respectively. Two repeated experiments were conducted to obtain the individual Tm values.