Fig. 2.

Loxl1 deficiency alters morphology and tropoelastin expression in young mouse lungs. (A) Representative images of Weigert's Resorcin-Fuchsin-stained sagittal lung sections showing expanded alveolar spaces in 2-month-old Loxl1^−/− BL/6, 50/50 and 129S mice. Scale bars: 100 μm (upper panels); 20 μm (lower panels). (B) The average alveolus area was quantified from five images from four mice/genotype/background at 20× magnification using particle analysis in Fiji. (C) Tropoelastin expression was determined by immunoblotting in lung tissue homogenates from BL/6, 50/50 and 129S mice (n=5-6 mice/genotype/background) and normalized to GAPDH expression. Bars show the mean±s.d. For B and C, ordinary one-way ANOVA followed by Tukey's multiple comparisons test was used for statistical analysis. *P<0.05; **P<0.01; ***P<0.001.