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. 2020 Dec 29;133(24):jcs245571. doi: 10.1242/jcs.245571

Fig. 3.

Fig. 3. Sec71 is localized to the center of the BFA body. Immunostaining and plots of signal intensities from wild-type (A,C,E,G) and Sec71F713Y S2 cells (B,D,F,H–J). Right, plots of signal intensities from images on the left. Signal intensities were measured along the 1.5 µm (upper panels) and 5 µm (lower panels) arrows shown in insets. In all panels, cells not treated with BFA are shown in the upper row and BFA-treated cells are shown in the lower row. 50 μM and 25 μM BFA was used for the wild-type cells and Sec71F713Y S2 cells, respectively. (A,B) Immunostaining with anti-Golgin245 (green), anti-GM130 (red), and anti-Sec71 (blue) antibodies. (C,D) Immunostaining with anti-Rab11 (green), anti-GM130 (red), and anti-Sec71 (blue) antibodies. (E,F) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) immunostained with anti-Sec71 (blue) antibody. (G,H) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab11 (red) immunostained with anti-Sec71 (blue) antibody. (I) S2 cells expressing ST::EGFP (green) immunostained with anti-GM130 (red) and anti-Rab6 (blue) antibodies. (J) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) immunostained with anti-Golgin245 (blue) antibody. Scale bars: 5 μm (A,C,E, left panels), 1 μm (A,C,E, right panels, B,D,F,G–J).

Sec71 is localized to the center of the BFA body. Immunostaining and plots of signal intensities from wild-type (A,C,E,G) and Sec71F713Y S2 cells (B,D,F,H–J). Right, plots of signal intensities from images on the left. Signal intensities were measured along the 1.5 µm (upper panels) and 5 µm (lower panels) arrows shown in insets. In all panels, cells not treated with BFA are shown in the upper row and BFA-treated cells are shown in the lower row. 50 μM and 25 μM BFA was used for the wild-type cells and Sec71F713Y S2 cells, respectively. (A,B) Immunostaining with anti-Golgin245 (green), anti-GM130 (red), and anti-Sec71 (blue) antibodies. (C,D) Immunostaining with anti-Rab11 (green), anti-GM130 (red), and anti-Sec71 (blue) antibodies. (E,F) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) immunostained with anti-Sec71 (blue) antibody. (G,H) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab11 (red) immunostained with anti-Sec71 (blue) antibody. (I) S2 cells expressing ST::EGFP (green) immunostained with anti-GM130 (red) and anti-Rab6 (blue) antibodies. (J) S2 cells expressing GalT::EGFP (green) and tdTomato::Rab6 (red) immunostained with anti-Golgin245 (blue) antibody. Scale bars: 5 μm (A,C,E, left panels), 1 μm (A,C,E, right panels, B,D,F,G–J).