TABLE 1.
Reactivities of serum polyclonal antibodies against culture supernatants of LAB strains as determined by NCI-ELISA, CI-ELISA, and CD-ELISA
| Microorganism (bacteriocin produced) | Sourcea | Cross-reactivity (%) in:
|
||
|---|---|---|---|---|
| NCI-ELISAb | CI-ELISAc | CD-ELISAc | ||
| Pediococcus acidilactici 347 (pediocin PA-1) | Our collection | 100 | 100 | 100 |
| Pediococcus acidilactici 347 Ped− (non-pediocin PA-1 producer) | Our collection | 1.6 | NRd | NR |
| Pediococcus pentosaceus FBB61 (pediocin A) | TNO | NR | NR | NR |
| Enterococcus faecium T136 (enterocins A and B) | Our collection | 2.3 | 1.9 | 1.7 |
| Enterococcus faecium P13 (enterocin P) | Our collection | 1.1 | 0.5 | 0.2 |
| Enterococcus faecium L50 (enterocins L50A and L50B) | Our collection | 1.1 | NR | NR |
| Enterococcus faecalis INIA4 (enterocin AS-48) | INIA | 1.3 | NR | NR |
| Lactobacillus sake 148 (lactocin S) | Our collection | 3.2 | 3.0 | NR |
| Lactococcus lactis BB24 (nisin A) | Our collection | 3.5 | 3.6 | 1.9 |
| Lactococcus lactis MG1614 (nonbacteriocin producer) | IFR | 2.3 | 1.9 | 2.7 |
IFR, Institute of Food Research, Norwich Laboratory (Norwich, United Kingdom); INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Madrid, Spain); TNO, Nutrition and Food Research (Zeist, The Netherlands).
Cross-reactivity was defined as [(absorbance reading above the limit of detection produced by a culture supernatant/absorbance reading above the limit of detection produced by a supernatant of P. acidilactici 347) × 100].
Cross-reactivity was defined as [(antibody binding inhibition produced by a culture supernatant/antibody binding inhibition produced by a supernatant of P. acidilactici 347) × 100].
NR, no reactivity.