Fig. 5.

MaR1 regulates the Keap1-Nrf2signaling axis. A The docking mode of maresin1 with keap1 (PDB code: 5CGJ). Maresin1 and the amino acid residues that participate in the interactions were displayed as sticks and colored by the element, hydrogen bonds are showed as green dashed lines. B Western blot detection of KEAP1 protein level. C BMDMs were treated with or without ML334 (1 μM) for 1 h, then stimulated with FAs + MSUc for 12 h. Western blot detection of p-NRF2 protein level. D Effect of Keap1 knockdown on p-NRF2 protein level. E and F ML334 treatment or Keap1 knockdown increased Prdx5 mRNA expression. *Compared with no FAs + MSUc treatment, # compared with FAs + MSUc treatment. * and # means P < 0.05