Fig. 6.

Prdx5 over-expression promotes AMPK activity. BMDMs were transfected with control (pcDNA3.1) or Prdx5 open reading frame (ORF) was cloned into pcDNA3.1 plasmids (Prdx5 OE) for 24 h and then stimulated with FAs + MSUc for 12 h. A Western blot analysis for p-AMPK, TXNIP, TRX1, TRX2 and p-DRP1 protein levels. B Immunofluorescence staining to detect the signal intensity of TRX2 protein. C BMDMs were stained with Mitotracker Red probe, then immunofluorescence staining for p-Drp1 (green). Scale bar: 40 μm. Areas outlined in white are shown enlarged in the right panels. Image is representative of 5 images/dish; n = 3 dishes/condition. D Representative TEM images of morphological changes in mitochondria (white box) in BMDMs. Scale bar: 1 μm. Image is representative of 10 images/sample; n = 3 samples/condition. E BMDMs were stained with Mitotracker Green probe and DAPI, and mitochondrial morphology was analyzed by LCM imaging. Image is representative of 5 images/dish; n = 3 dishes/condition. *Compared with no FAs + MSUc treatment, # compared with FAs + MSUc treatment. * and # means P < 0.05