Fig. 2.

Parasponia andersonii SYMRK is essential for mycorrhization and nodulation. A Nodule numbers formed in P. andersonii empty vector control line (EV) and three Pansymrk mutant lines, 6 weeks post-inoculation with Mesorhizobium plurifarium BOR2. B mycorrhization efficiency in the root system of P. andersonii EV-control and three independent Pansymrk mutant lines 6 weeks post-inoculation with Rhizophagus irregularis DOAM197198. F%: The frequency of mycorrhiza in the infected root system. M%: the intensity of mycorrhizal colonisation in the infected root system. A%: Arbuscule abundance in the infected root system. a%: Averaged arbuscule abundance detected in 50 randomly selected 1 cm segments of a root system. Error bars represent the SE of 10 biological replicates, for each 50 × 1 cm root segment that has been analyzed. Analysis was done according to Trouvelot et al. (1986) [26] (C-F): Toluidine blue-stained P. andersonii EV-control C, Pansymrk-4 D, Pansymrk-5 E, and Pansymrk-6 F root segment visualizing R. irregulates infections 6 weeks post-inoculation. Size bar = 10 μm