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. 2023 Nov 24;23:587. doi: 10.1186/s12870-023-04594-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — PanSYMRK ectopic expression induces spontaneous nodulation in Parasponia andersonii. (A, B) Bright-field A and green fluorescent image B of P. andersonii A. rhizogenes-transformed roots expressing GFP and PanSYMRK under control of the pLjUBI1 promoter showing spontaneously formed nodule-like structures (6 weeks post planting). C Relative gene expression of PanSYMRK in P. andersonii A. rhizogenes-transformed roots containing an empty vector (EV) or pLjUBI1:PanSYMRK (n = 3). D Longitudinal section of a spontaneously formed nodule-like structure visualizing cortical and pericycle cell divisions