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. 2023 Nov 24;23:587. doi: 10.1186/s12870-023-04594-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Parasponia symrk-5 mutant trans-complementation of root nodule symbiosis. A Schematic representation of P. andersonii SYMRK gene structure. Arrowhead points to the location of the introduced GA mutation in PanSYMRK at the 5’-donor splice site of intron 12. B Nodule number per plant formed on Pansymrk-5 A. rhizogenes transformed root with pPanSYMRK:PanSYMRK gene (n = 13). (C-E) Nodule number per plant (n = 5) C, representative image of green flurescence protein (GFP) nodule D and a section through a mature nodule E of Pansymrk-5 A. rhizogenes transformed root with pPanSYMRK:PanSYMRK^GA carrying a GA mutation at the 5’-donor splice site of intron 12. Nodules were harvested and analysed at 8 weeks post inoculation with Mesorhizobium plurifarium BOR2 (OD600 = 0.025)