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. 2023 Nov 24;23:587. doi: 10.1186/s12870-023-04594-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Phylogeny of SYMRK including the splice site dinucleotide motifs for intron 12. Phylogeny was reconstructed based on an alignment of SYMRK orthologous proteins from 19 species. Leaves are labelled by their respective species, gene name if available) and gene identifier. The non-canonical GC donor splice site is common in SYMRK intron 12 of dicotyledon species, except in Glycine max SYMRKβ and Pisum sativum SYM19, where GC is substituted by GT. In contrast, only Trema orientalis RG33 possesses a GA motif in this position (highlighted in red)