Table 2.
Clinical indication for bone and/or joint biopsy and microbiological results of traditional cultures and NGS.
| Case | Clinical Indications | Traditional Cultures | NGS | Concordance Methods | Discordance Methods |
|---|---|---|---|---|---|
| 1 | Tibial osteomyelitis | Pseudomonas aeruginosa | Pseudomonas aeruginosa | x | |
| 2 | PJI | Negative | Negative | x | |
| 3 | Septic arthritis + omeral osteomyelitis | Staphylococcus epidermidis | Staphylococcus epidermidis | x | |
| 4 | PJI | Staphylococcus aureus | Staphylococcus aureus | x | |
| 5 | Tibial osteomyelitis with hardware | Negative | Cutibacterium acnes | x | |
| 6 | Septic arthritis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 7 | PJI | Staphylococcus aureus | Staphylococcus aureus | x | |
| 8 | PJI | Chryseobacterium spp. | Chryseobacterium spp. | x | |
| 9 | PJI | Negative | Negative | x | |
| 10 | PJI | Staphylococcus epidermidis | Cutibacterium spp. | x | |
| 11 | Tibial osteomyelitis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 12 | Tibial osteomyelitis | Corynebacterium coyleae | Bacillus spp. | x | |
| 13 | Femoral osteomyelitis with hardware | Staphylococcus capitis | Staphylococcus capitis | x | |
| 14 | PJI | Negative | Negative | x | |
| 15 | Foot bursitis | Negative | Negative | x | |
| 16 | PJI | Staphylococcus aureus | Cutibacterium acnes | x | |
| 17 | PJI | Enterococcus faecalis | Negative | x | |
| 18 | PJI | Bacillus spp. | Bacillus spp. | x | |
| 19 | Femoral osteomyelitis | Negative | Negative | x | |
| 20 | PJI | Negative | Streptococcus pyogenes | x | |
| 21 | Sacral osteomyelitis | Negative | Negative | x | |
| 22 | PJI | Negative | Burkholderia spp. | x | |
| 23 | Tibial osteomyelitis with hardware | Staphylococcus aureus | Negative | x | |
| 24 | Wrist osteomyelitis | Staphylococcus aureus | Negative | x | |
| 25 | Foot osteomyelitis | Staphylococcus epidermidis | Staphylococcus epidermidis | x | |
| 26 | Septic arthritis | Negative | Negative | x | |
| 27 | PJI | Negative | Negative | x | |
| 28 | PJI | Staphylococcus haemolyticus | Negative | x | |
| 29 | Femoral osteomyelitis with hardware | Pseudomonas aeruginosa | Pseudomonas aeruginosa | x | |
| 30 | Foot osteomyelitis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 31 | Septic arthritis | Negative | Cutibacterium acnes | x | |
| 32 | Foot osteomyelitis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 33 | Foot osteomyelitis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 34 | PJI | Staphylococcus aureus | Staphylococcus aureus | x | |
| 35 | Septic arthritis | Negative |
Finegoldia magna, Cutibacterium acnes |
x | |
| 36 | Foot osteomyelitis | Corynebacterium striatum | Corynebacterium striatum, Bacteroides pyogenes | x | |
| 37 | PJI | Negative | Cutibacterium acnes | x | |
| 38 | Tibial osteomyelitis with hardware | Staphylococcus aureus | Cutibacterium acnes | x | |
| 39 | PJI | Negative | Negative | x | |
| 40 | Spacer infection | Staphylococcus aureus | Staphylococcus aureus | x | |
| 41 | PJI | Staphylococcus epidermidis | Staphylococcus epidermidis | x | |
| 42 | Septic arthritis | Negative | Staphylococcus hominis | x | |
| 43 | PJI | Staphylococcus epidermidis | Staphylococcus epidermidis | x | |
| 44 | PJI | Negative | Staphylococcus epidermidis | x | |
| 45 | Septic osteonecrosis | Negative | Cutibacterium acnes | x | |
| 46 | PJI | Negative | Finegoldia magna | x | |
| 47 | PJI | Negative | Cutibacterium acnes | x | |
| 48 | Tibial osteomyelitis | Staphylococcus aureus | Staphylococcus aureus | x | |
| 49 | PJI | Staphylococcus epidermidis | Staphylococcus epidermidis | x | |
| Total | 30/49 (61%) | 19/49 (39%) |
NGS, next-generation sequencing; PJI, prosthetic joint infection.