FIG. 2.

Schematic representation of the integrations of the tetracycline resistance cassette into the pepX gene (A) and of the plasmid pTIL102 into the pepP gene (B). Both genes are indicated by empty bars, and the internal fragment of the pepP gene used for the integration is shown by a dotted bar. The arrows in plasmids pTIL18B and pTIL102 indicate the E. coli thermosensitive origin of pG⁺host4 and the E. coli origin of replication of the pTAg plasmid, respectively. The relevant phenotypes of the clones are indicated on the right. H, HindIII restriction site; B, BglII restriction site.