Fig. 3.
Confirmation of differentially expressed genes identified by RNAseq analyses in B. burgdorferi infected MDA-MB-231 and MCF10A cell lines by qRT-PCR. 1 × 106 cells of MDA-MB-231 and MCF10A cell lines were infected with B. burgdorferi for 48 h, RNA was extracted, cDNA was synthesized, and subjected to qRT-PCR as discussed in Material and Methods. The expression fold change is normalized to GAPDH, and the delta-delta Ct method is used for quantitative analyses. (A) qRT-PCR and RNA-seq results for eight genes were differentially expressed in MDA-MB-231 while (B) qRT-PCR and RNA-seq results for nine genes were differentially expressed in MCF10A cells upon B. burgdorferi infection