Fig. 3.

Immunohistochemical detection of the presence of pyruvate carboxylase (PC), pyruvate dehydrogenase (PDH) and glial fibrillary acidic protein (GFAP) in human brain section. The expression of PC, PDH and GFAP in the cells was immunofluorescently labeled with primary anti-PC rabbit polyclonal antibodies, anti-PDH human polyclonal antibodies and anti-GFAP mouse monoclonal antibodies followed by affinity-purified anti-rabbit secondary antibodies conjugated to Alexa Fluor 488 (green), anti-human secondary antibodies conjugated to TRITC (orange) and anti-mouse secondary antibodies conjugated to Alexa Fluor 633 (red). Nuclei were visualized using DAPI intercalating fluorochrome (DAPI; blue). The merged views of the PC, PDH, GFAP and DAPI are represented in merge (yellow). Negative control was made by omitting primary antibodies (negative control). The scale bars represent 20 μm and in bottom merge represents 10 μm.