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. 2023 Oct 26;12(11):1914. doi: 10.3390/antiox12111914

Figure 12.

Figure 12

ZYAN1 enhances expressions of autophagy-related proteins and promotes the formation of autophagosome at P28. (A) TEM examination showed that the mitochondrial damage was ameliorated by ZYAN1 treatment, as evidenced by increased number of autophagosomes and reduced number of autophagy vacuoles. The black arrows represent autophagosomes; the yellow triangle represents autophagy vacuoles. (B) Western blot showed that the expression levels of ATG5, Beclin-1, and LC3-B in the retina of RD + ZYAN1 group increased at P28. (C) The expression level of P62 in RD + ZYAN1 group was significantly lower than that in RD model (D,E) Western blot showed that the expression levels of HIF-1α and BNIP3 protein in the retina of RD + ZYAN1 group increased compared with those in the RD group (ns, ** p < 0.01, *** p < 0.001, **** p < 0.0001 for differences compared with normal control group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 for differences compared with RD + ZYAN1 group, n = 6).