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. 2023 Oct 28;11(11):2915. doi: 10.3390/biomedicines11112915

Figure 5.

Figure 5

The E165Q mutant can be cleaved by co-transfected wildtype CLCA2 in a concentration-dependent manner. Left, immunoblot analysis of the co-transfected wildtype (WT) and E165Q (EQ) mutant anti-Flag immunoprecipitates from 1% NP40 lysates. Cleavage of the mutant protein is indicated by the 35 kDa band in lanes 4–6. Lanes 1–2 show level of cleavage product from cells transfected with EQ alone or WT alone; lane 3, nontransfected. Right, relation between percent cleavage of the EQ and amount of co-transfected wildtype CLCA2; * p-value < 0.05; ** p-value < 0.005. The percent cleavage of WT-Flag in lane 2 is 55. Experiment repeated three times.