Figure 6.

Co-immunoprecipitation of CLCA2 precursors. (A) Immunoblots of lysates from co-transfected or singly transfected HEK293T cells that were subjected to immunoprecipitation with anti-Flag or anti-Myc antibodies. NT indicates N-terminal insertion of the Myc tag. CT indicates C-terminal insertion of the Flag tag. The Myc antibody immunoprecipitates only the Flag-tagged precursor but not the C-terminal product. (B) E165Q mutation does not affect ability to form a stable complex. Immunoblot analysis of co-immunoprecipitation from lysates of HEK293T cells transfected with Myc-tagged wildtype CLCA2 and/or Flag-tagged CLCA2 E165Q mutant. On both gels, lane 2 was left empty to avoid spillover. Experiments were repeated three times.