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Accumulation of trehalose in trehalose-deficient and/or hsp104 mutant cells during heat shock treatment. Logarithmic-phase cells of the wild-type strain (CWG13 [W]), the trehalose-deficient mutant (CWG14 [T]), the hsp104 mutant (CWG15 [104]), and the double mutant (CWG12 [D]), were incubated at 43°C, and trehalose accumulation was estimated at the indicated times. The error bar for each sample represents the SD of at least three independent experiments.
