FIG. 3.

Neutral trehalase activities in trehalose-deficient and/or hsp104 mutant cells. Logarithmically growing (L) and heat-shocked (H) cells of the wild type (CWG13 [W]), the trehalose-deficient mutant (CWG14 [T]), the hsp104 mutant (CWG15 [104]), and the double mutant (CWG12 [D]) were harvested, and crude extracts were prepared. The enzyme activities of neutral trehalase were measured in the crude extracts as described in the text. The error bar for each sample represents the SD of at least three independent experiments.