FIG. 4.

Activities of enzymes of trehalose metabolism in hsp104 mutant cells. Logarithmically growing (L) and heat-shocked (H) cells of the wild-type strain (W303aLEU2+) and the hsp104 mutant (Δ104LEU2) were harvested, and crude extracts were prepared. The enzyme activities of neutral trehalase (A), trehalose-6-phosphate (T6P) synthase (B), and trehalose-6-phosphate phosphatase (C) were measured in crude extracts as described in the text. The error bar for each sample represents the SD of at least three independent experiments.