TABLE 2.
Plaque assays on transconjugants with six lytic phagesa
| L. lactis strain | Plasmid content | Bacterio-phage | No. of plaques | Plaque size (mm) | EOP |
|---|---|---|---|---|---|
| MG1614 | None | 712 | 1.2 × 109 | 0.6 | 1.0 |
| sk1 | 7.5 × 108 | 0.6 | 1.0 | ||
| eb1 | 1.3 × 109 | 0.7 | 1.0 | ||
| c2 | 3.2 × 109 | 3.5 | 1.0 | ||
| ml3 | 1.0 × 1010 | 3.6 | 1.0 | ||
| stl1 | 6.8 × 108 | 2.7 | 1.0 | ||
| DPC5013 | pLP712 | 712 | 1.3 × 109 | 0.6 | 1.1 |
| sk1 | 7.5 × 108 | 0.65 | 1.0 | ||
| eb1 | 1.5 × 109 | 0.65 | 1.2 | ||
| c2 | 3.9 × 109 | 3.4 | 1.2 | ||
| ml3 | 9.6 × 109 | 3.8 | 1.0 | ||
| stl1 | 6.1 × 108 | 2.6 | 0.9 | ||
| DPC3343 | pAH82 | 712 | 4.0 × 107 | 0.55 | 3.3 × 10−2 |
| sk1 | 8.0 × 107 | 0.55 | 1.1 × 10−1 | ||
| eb1 | 1.6 × 109 | 0.7 | 1.2 | ||
| c2 | 2.7 × 109 | 3.2 | 0.8 | ||
| ml3 | 1.2 × 1010 | 3.4 | 1.2 | ||
| stl1 | 7.3 × 108 | 2.4 | 1.1 | ||
| DPC3290 | pAH90 | 712 | 5.0 × 106 | 0.6 | 4.2 × 10−3 |
| sk1 | 8.4 × 107 | 0.65 | 1.1 × 10−1 | ||
| eb1 | — | — | — | ||
| c2 | — | — | — | ||
| ml3 | — | — | — | ||
| stl1 | — | — | — | ||
| DPC5012 | pMRC01 | 712 | — | — | — |
| sk1 | — | — | — | ||
| eb1 | — | — | — | ||
| c2 | 2.8 × 109 | 0.6 | 0.9 | ||
| ml3 | 9.8 × 109 | 0.6 | 1.0 | ||
| stl1 | — | — | — | ||
| DPC5002 | pMRC01, pAH82 | 712 | — | — | — |
| sk1 | — | — | — | ||
| eb1 | — | — | — | ||
| c2 | 3.4 × 109 | 0.65 | 1.1 | ||
| ml3 | 9.6 × 109 | 0.6 | 1.0 | ||
| stl1 | — | — | — | ||
| DPC5001 | pMRC01, pAH90 | 712 | — | — | — |
| sk1 | — | — | — | ||
| eb1 | — | — | — | ||
| c2 | — | — | — | ||
| ml3 | — | — | — | ||
| stl1 | — | — | — | ||
| DPC5000 | pMRC01, pAH90, pLP712 | 712 | — | — | — |
| sk1 | — | — | — | ||
| eb1 | — | — | — | ||
| c2 | — | — | — | ||
| ml3 | — | — | — | ||
| stl1 | — | — | — |
a Plaque data are the averages of three separate experiments. Dashes indicate that no plaques formed. Phages 712 and sk1 belong to the 936/P008 group, and eb1, c2, ml3, and stl1 belong to the c2/c6A group.