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. 2023 Nov 6;13(11):1622. doi: 10.3390/biom13111622

Table 1.

Studies that summarized the involvement of lncRNAs in hypoxic/anoxic conditions.

Studies that Summarized the Involvement of LncRNAs SNHG Family in Hypoxic/Anoxic Conditions
SH-SY5Y OGD/R-induced SNHG1 The overexpression of SNHG1 or the KO of miR-140-5p alleviated hypoxia-induced damage. Overall, the results proved that lncRNA SNHG1 could be novel therapeutic target in hypoxic brain injury [63]
Primary BMEC mice OGD/R-induced SNHG1 Snhg1 silencing led to worsening the BMEC apoptosis that was OGD/R-induced, so confirmed the protective effect of lncRNA Snhg1in in IS model [64]
C57Bl/6 mice with ligation of the right internal carotid artery.
Primary hippocampal cell culture with shRNA or miRNA mimic transfection
SNHG3 Snhg3 silencing led to reduction in cell viability and promoted the apoptosis, in vivo
Snhg3 overexpression improved brain neurological function in the animals and reduced HIBD
[65]
OGD/R-induced primary neurons SNHG12 SNHG12 knockdown exacerbated apoptosis induced by OGD/R [66]
HT22 cells of I/R model SNHG12 SNHG12 increased cell activity and inhibited oxidative stress through inhibition of SIRT1/FOXO3a signaling-mediated autophagy [67]
N2A cells ODG/R-induced
MCAO/R mice
SNHG14 SNHG14 silencing led to an increase in miR-98-5p and BCL2L13 depletion. So, SNHG14 is beneficial to OGD/R [68]
MCAO male SD rats
OGD-induced
primary cortical neurons of SD embryos rat
SNHG14 SNHG14 silencing led to a reduction in the infarct volume and improved neurological deficits in MCAO rats [69]
H/I mice model
PC12 cells OGD-stimulated
SNHG15 SNHG15 suppression alleviated H/I brain injury by modulating the miR-153-3p/SETD7 axis [70]
HBMECs OGD/R-induced SNHG16 The overexpression of SNHG16 led to a decrease in miR-15a-5p and increased the Bcl-2 expression levels, as well as reduced apoptosis and enhanced cell survival [71]
Studies that Summarized the Involvement of LncRNA MALAT1 In Hypoxic/Anoxic Conditions
C57BL/6 mice with right common carotid artery occlusion
C57BL/6 mice hippocampal neurons with H/I
MALAT1 MALAT1 acting as a miR-429 sponge led to an increment in WNT1 expression promoting apoptosis, as shown by expression levels of cleaved caspase-3 and the ratio of Bax to Bcl-2 both in vitro and vivo [72]
HBMECs OGD-induced MALAT1 Through sponging miR-126, MALAT1 inhibited proliferation and induced apoptosis of OGD-induced HBMECs [73]
bEnd.3 OGD-induced MALAT1 The MALAT1-miR-200c-3p-SIRT1 pathway could be interesting to modulate for influencing autophagy and cell survival for neuroprotection [74]
BMECs of C57BL/6J mice OGD/R-induced MALAT1 MALAT1 increased autophagy and cell survival in BMECs [75]
MCAO mice
BMECs OGD-induced
MALAT1 MALAT1 interactions reduced cell death and inflammation [76]
MCAO C57BL/6J mice
BMECs OGD/R-induced
MALAT1 Following OGD/R, MALAT1 regulated angiogenesis [77]
Studies that Summarized the Involvement of LncRNA H19 In Hypoxic/Anoxic Conditions
Neonatal HIE SD rats H19 H19 overexpression reduced both the nervous damage and autophagy of brain tissue, as well as improved neurological functions [78]
Primary neuron cells rat OGD-induced
HIBD SD rat induced by partial occlusion of carotid artery
H19 HIBD can be prevented by overexpression of the lncRNA H19 [79]
HIBD neonatal rats H19 H19 overexpression suppressed apoptosis in rat cardiomyocytes [80]
Blood samples of IS patients
MCAO/R rats
SH-SY5Y cells OGD-induced
H19 Hypoxia/ischemia-induced neuronal injury was attenuated by blocking the lncRNA H19-miR-19a-Id2 axis [81]
PC-12 cells induced by hypoxia H19 Overexpression of lncRNA H19 led to an increase in cell damage induced by hypoxic conditions [82]
IS patients
MCAO mouse
BV2 cell OGD-induced
H19 H19 could be a useful marker as well as a potential therapeutic target for IS patients [83]
Studies that Summarized the Involvement of LncRNA GAS5 in Hypoxic/Anoxic Conditions
Blood of CA/CCR patients
OGD/R-inducted astrocytes
GAS5 Inhibiting PI3K/Akt signaling through INPP4B, lncRNAGAS5/miR-137 is a hypoxia-responsive axis involved in astrocyte–microglia crosstalk. [84]
HIBD neonatal rat model established and treated with shRNA-GAS5 or antagomir miR-128-3p GAS5 Through the microRNA-128-3p/Bax/Akt/GSK-3β axis, downregulation of GAS5 prevents mitochondrial apoptosis and HIBD in neonatal rats [85]
Primary rat cortical cell hypoxia-treated
B35 cell with hypoxic treated
MCAO SD rats
GAS5 GAS5 can lead to worsening of cell apoptotic processes in hypoxic conditions targeting miR-221/PUMA axis. Consequently, GAS5 could be useful in ischemic stroke treatment [86]
Studies that Summarized the Involvement of LncRNA NEAT1 in Hypoxic/Anoxic Conditions
BMECs OGD-induced NEAT1 NEAT1 facilitates survival and angiogenesis in OGD-induced BMECs via targeting miR-377 [87]
Neonatal HIBD mouse model was established via right common carotid artery occlusion NEAT1 The findings demonstrated that NEAT1 alleviated HIBD in mice by binding to miR-339-5p [88]
Studies that Summarized the Involvement of LncRNA MIAT in Hypoxic/Anoxic Conditions
Neuro2A cells OGD-induced
Neonatal rat with permanent unilateral carotid ligation
MIAT The overexpression of MIAT reduced neuron apoptosis and relieved HI injury of neonatal rats through miR-211/GDNF [89]
MCAO SD rats
Primary rat neurons OGD/R- stimulated
MIAT MIAT exacerbated I/R injury by disrupting redox homeostasis in neurons [90]
Studies that Summarized the Involvement of LncRNA PVT1 in Hypoxic/Anoxic Conditions
MCAO mice
Primary neurons OGD/R-induced
PVT1 PVT1 silencing led to a reduction in infarct volume and improved neurological behavior in MCAO mice [91]
HUVECs OGD-induced PVT1 PVT1 alleviated hypoxia-induced endothelial apoptosis by enhancing autophagy through the miR-15b-5p/ATG14 and miR-424-5p/ATG14 pathways [92]
Studies that Summarized the Involvement of LncRNA Peg13 in Hypoxic/Anoxic Conditions
Neonatal HIBD mice
Mouse hippocampal neurons OGD-induced
PEG13 Peg13 exerted an anti-apoptotic role, acting as a sponge for miR-20a-5p targeting XIAP [93]
bEnd.3 cells OGD/R-induced
MCAO mice
PEG13 Peg13 alleviated I/R-induced neurological deficit, cerebral infarct, and cerebral edema [94]
Studies that Summarized the Involvement of LncRNA MEG3 in Hypoxic/Anoxic Conditions
PC12 cells induced by hypoxia MEG3 MEG3 silencing performed by siRNA played a neuroprotective function, preventing hypoxia-induced injury in PC12 via the modulation of protein involved in apoptosis and cell proliferation [95]
MCAO C57BL/6J mice MEG3 Silencing MEG3 resulted in being protective against I/R-induced ischemic damage in vivo and improved overall neurological functions [96]
PC12 cells cultivated in hypoxic conditions MEG3 MEG3 led to an increase in hypoxic damage in PC12 cells by targeting miR147 and the downstream target Sox2 [97]
260 neonatal female or male C57/BL6 mice with right cervical vessels ligated with a double bandage MEG3 MEG3 silencing improved the therapeutic effect of DEX on HIBD in neonatal mice through the expression of miR-129-5p [98]
Studies that Summarized the Involvement of LncRNA BDNF-AS in Hypoxic/Anoxic Conditions
Mice received ligation of the unilateral common carotid artery
Primary hippocampal neuron with hypoxic and oxidative stress induced
BDNF-AS Silenced BDNF-AS lncRNA attenuated HI events [99]
HCN2 and human astrocytes after H/R BDNF-AS lncRNA BDNF-AS knockdown suppressed apoptosis [100]

OGD/R: oxygen-glucose deprivation/reperfusion; KO: knockout; BMEC: brain microvascular endothelial cells; IS: ischemic stroke; HIBD: hypoxic ischemic brain damage; I/R: ischemic/reperfusion; MCAO/R: middle cerebral artery occlusion/reperfusion; MCAO: middle cerebral artery occlusion; SD: Sprague Dawley; H/I: hypoxic ischemic; HBMECs: human brain microvascular endothelial cells; Bcl-2: B-cell lymphoma 2; MALAT1: Metastasis-Associated Lung Adenocarcinoma Transcript 1; bEnd.3: brain microvascular endothelial cells; OGD: oxygen-glucose deprivation; HIE: hypoxic-ischemic encephalopathy; CA/CCR: Cardiac Arrest/Cardiopulmonary Cerebral Resuscitation; GAS5: Growth Arrest-Specific 5; PUMA: p53-upregulated modulator of apoptosis; NEAT1: Nuclear paraspeckle assembly transcript 1; MIAT: myocardial Infarction Associated Transcript; PVT1: plasmacytoma variant translocation 1; HUVECs: human umbilical vein endothelial cells; ATG14: autophagy-related 14; XIAP: X chromosome-linked inhibitor of apoptosis; MEG3: Maternally expressed gene 3; DEX: dexmedetomidine; and HCN2: human cortical neurons.