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. Author manuscript; available in PMC: 2024 Nov 22.
Published in final edited form as: Cell. 2023 Nov 22;186(24):5375–5393.e25. doi: 10.1016/j.cell.2023.10.019

Figure 6. Neuronal PAR1 (F2r) is required for V8 and S. aureus-induced itch.

Figure 6.

(A) PBS or V8 protease injected intradermally into cheek of wildtype (F2r+/+) and F2r−/− mice, spontaneous scratching over 30 min. (n=3-4 males, 2-4 females per group)

(B) Acute itch behaviors measured for mice treated with vehicle, control siRNA, or F2r siRNA (n=4-6 males, 4-6 females per group).

(C-F) Mice receiving intrathecal siRNA injections were treated with PBS or exposed to MRSA. Spontaneous itch (D), alloknesis, (E) and scratch-induced skin damage (F) measured 5-days post-exposure (n=4-6 males per group).

(G) Generation of Trpv1ΔF2r and Trpv1cre− control mice.

(H-I) Spontaneous itch (H) and alloknesis (I) for Trpv1ΔF2r and control mice treated with PBS or exposed to MRSA (n=3-8 males, 1-6 females per group).

For each panel, data combined from 2-3 independent experiments are shown. Data are represented as mean±SD.

Statistical analysis: (A, D-F, H, I) Two-way ANOVA, Sidak’s multiple comparisons. (B, E) Mann-Whitney test. **P<0.01; ***P<0.001; ****P<0.0001; ns, not significant. See also Figure S8.