Table 1.

Summary of decellularization methods.

E protocol		D protocol
Solution	Temperature, Time	Solution	Temperature, Time
Freeze-thaw	−20 °C	Freeze-thaw	−20 °C
0.25 % Trypsin-EDTA	4 °C, 24h	TRITON™ X-100 1:100 in dH2O	4 °C, 24h
0.25 % Trypsin-EDTA	37 °C, 3h	dH2O	4 °C, 2 × 10min
dH2O	20 °C, 2 × 10min	SDS 1 %	20 °C, 24h
DNase I 200μg/ml	37 °C, 3h	DNase I 200μg/ml	37 °C, 3h
dH2O	4 °C, 72h	dH2O	4 °C, 72h