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SDS-PAGE. Lane 1, protein molecular weight standard; lane 2, E. coli E509 containing pRSET; lane 3, uninduced E. coli E509 containing pRSET-ptsH gene construct; lane 4, IPTG-induced E. coli E509 containing pRSET-ptsH gene construct; lane 5, purified L. monocytogenes HPr. kD, kilodaltons.
