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. 2023 Oct 27;45(11):8633–8651. doi: 10.3390/cimb45110543

Figure 5.

Figure 5

miR-1203 targeted and regulated the expression of ANT2. (A) Volcano map showing the 145 miRNAs commonly differentially expressed from the two datasets; the green dots represent miR-1203 (log2FC < −3, low expression). (B) Schematic representation of predicted miR-1203 binding sites on 3′UTR of ANT2 in humans. (C) Schematic representation of wild-type and mutated ANT2 3′UTR site. (D) AC16 cells transfected with wild-type or mutated ANT2 3′UTR luciferase constructs and control mimic or miR-1203 mimics; n = 3 per group. (E,F) ANT2 protein expression by Western blot analysis and gray analysis in AC16 cells transfection with miR-1203 mimics; n = 3 per group. * p < 0.05, ** p < 0.01, ns: no significance.

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