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. 2023 Nov 7;15(22):5309. doi: 10.3390/cancers15225309

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Combined analysis of RNA-seq and Circle-seq data. (A). Flow chart of the potential functional eccDNA analysis strategy (the upper panel), genes that harbored eccDNA in at least three tumor samples were indicated (the lower panel). (B). Validations for the expression of the eccDNA related to BAIAP2L2 detected in #83T, using PCR followed by gel electrophoresis and Sanger sequencing. (C). Genome track with paired-end reads from Circle-seq meeting the standards described above. The distribution of eccDNA reads related to the two indicated genes including SLC16A3 and BAIAP2L2 in #24T, 28T and 77T are shown.