Coomassie blue-stained PAGE gel containing purified C. thermophilium laccase. Lane M contained standard molecular mass protein markers, including phosphorylase b (molecular mass, 104 kDa), bovine serum albumin, (82 kDa), ovalbumin (48.3 kDa), carbonic anhydrase (33.4 kDa), and soybean trypsin inhibitor (28.3 kDa). Lane 1 contained 10 μg of purified C. thermophilium laccase.