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Purification of laccase from C. thermophilium
| Step | Vol (ml) | Protein concn (μg/ml) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Culture supernatant | 1,300 | 4 | 22 | 3.6 | 100 | |
| Ultrafiltration | 110 | 16 | 19 | 11 | 84 | 3 |
| DEAE | 26 | 36 | 17 | 19 | 79 | 5 |
| Concanavalin A | 1.2 | 190 | 8.4 | 37 | 40 | 10 |
