TABLE 2.
Kinetic parameters of C. thermophilium laccase for oxidation of various substrates (obtained by measuring the oxygen consumption rates with 10 μg of purified enzymea
| Substrateb | Km (mM) | Vmax (μM O2 min−1) |
|---|---|---|
| ABTS | 0.19 | 2.6 |
| l-DOPA | 0.10 | 1.9 |
| 2,6-DMP | 0.096 | 3.4 |
| Ferulic acid | 0.27 | 3.8 |
| Gallic acid | 0.13 | 5.0 |
| Guaiacol | 0.40 | 2.5 |
| Hydroxyquinone | 0.036 | 1.7 |
| Syringaldazine | 0.034 | 4.1 |
| Vanillic acid | 0.15 | 2.1 |
a
All values are the means of duplicate measurements, and the coefficients of variation were less than 5%.
b
ABTS, 2,2′-azinobis(3-ethylbenz-thiazoline-6-sulfonic acid); l-DOPA, l-3,4-dihydroxyphenylalanine; 2,6-DMP, 2,6-dimethoxyphenol.