TABLE 3.
Inhibition of laccase purified from C. thermophilium
| Inhibitor | Concn (mM) | % Inhibitiona |
|---|---|---|
| Sodium azide | 0.01 | 12 |
| 0.05 | 84 | |
| 0.1 | 96 | |
| 10 | 96 | |
| EDTA | 1 | 34 |
| 2 | 39 | |
| 5 | 67 | |
| 10 | 95 | |
| 25 | 100 | |
| Thioglycolic acid | 0.1 | 91 |
| 0.2 | 94 | |
| 1.0 | 100 |
All values are the means of triplicate measurements, and the coefficients of variation were less than 5%; 100% activity was the activity obtained when 17-U/ml assay mixture containing 0.1 mM syringaldazine as a substrate was used.