TABLE 3.
Inhibitor | Concn (mM) | % Inhibitiona |
---|---|---|
Sodium azide | 0.01 | 12 |
0.05 | 84 | |
0.1 | 96 | |
10 | 96 | |
EDTA | 1 | 34 |
2 | 39 | |
5 | 67 | |
10 | 95 | |
25 | 100 | |
Thioglycolic acid | 0.1 | 91 |
0.2 | 94 | |
1.0 | 100 |
All values are the means of triplicate measurements, and the coefficients of variation were less than 5%; 100% activity was the activity obtained when 17-U/ml assay mixture containing 0.1 mM syringaldazine as a substrate was used.