FIG. 4.

SDS-PAGE of different fractions of G. sulfurreducens extracts. The gels were stained for protein with silver (A) and heme (B). Lanes 1, whole cells after KCl extraction; lanes 2, KCl extract from whole cells; lanes 3, isolated band from a preparative iron(III) reductase activity gel; lanes 4, molecular mass standards including phosphorylase b (97.4 kDa), bovine serum albumin (67 kDa), egg albumin (45 kDa), carbonic hydrase (29 kDa), soybean trypsin inhibitor (21 kDa), horse heart cytochrome c (12.5 kDa), and trypsin inhibitor (6.5 kDa).