TABLE 2.
Titration of free thiols from cells treated with 100 μM SNP and fractionated to give cell wall, membrane, and cytoplasma
| Fraction | μmol of SH (g of protein−1)
|
% Decrease | |
|---|---|---|---|
| Control cells | Cells treated with 100 μM SNP | ||
| Cell wall | 90 | 9 | 90 |
| Membrane | 60 | 53 | 10 |
| Cytoplasm | 157 | 86 | 50 |
a
C. sporogenes was grown in 3 liters of medium to give an optical density value at 550 nm of 1; SNP was then added to give a final concentration of 100 mM. The cells were harvested and fractionated into cell wall, membrane, and cytoplasm. For each fraction, the amount of free thiols was estimated as described in Materials and Methods.