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. 2023 Nov 9;24(22):16138. doi: 10.3390/ijms242216138

Table 2.

Condition for stable and transient transfection.

Cell Line Cells per Well in
6-Well Plate		 Transfection
Reagent		 Hygromycin
Concentration		 Amount of Plasmid Recommended
for Transient Transfection
CHO-K1 2 × 105/mL PEI 800 µg/mL Not tested
HEK293T 2 × 105/mL PEI 200 µg/mL Not tested
HeLa 2 × 105/mL PEI 200 µg/mL 0.5 µg
N2a 3,5 × 105/mL Genius 200 µg/mL 1 µg
S2-DGRC 2 × 106/mL Genius 200 µg/mL Not tested