Table 3.
Top scoring protein matches for proteins found within the cytoplasmic ~75 kDa and nuclear ~85 kDa protein bands detected as differentially glycosylated in VVA lectin blots of Galnt17−/− and wild type cerebellar proteins.
| SwissProt Accession | Score | Number of significant matches | Number of significant sequences | emPAIa | Description/Gene name |
|---|---|---|---|---|---|
| Non-nuclear 75kDa- Wild type | |||||
| SYN1_MOUSE | 515 | 23 | 10 | 0.78 | Synapsin-1/ Syn1 |
| K2C1_MOUSE | 201 | 6 | 2 | 0.14 | Keratin/ Krt1 |
| K1C10_MOUSE | 183 | 5 | 4 | 0.34 | Keratin/ Krt10 |
| Non-nuclear 75kDa- Galnt17−/− | |||||
| SYN1_MOUSE | 922 | 40 | 14 | 1.22 | Synapsin-1/ Sny1 |
| DPYL1_MOUSE | 416 | 14 | 6 | 0.72 | Dihydropyrimidinase-related protein 1/ Crmp1 |
| K1C10_MOUSE | 365 | 10 | 5 | 0.44 | Keratin/ Krt10 |
| Nuclear 85kDa- Wild type | |||||
| HS90B_MOUSE | 2929 | 85 | 23 | 3.36 | Heat shock protein HSP 90-beta/ Hsp90ab1 |
| HS90A_MOUSE | 2755 | 72 | 22 | 2.48 | Heat shock protein HSP 90-alpha/ Hsp90aa1 |
| ACON_MOUSE | 738 | 31 | 15 | 1.10 | Aconitate hydratase/ Acon |
| Nuclear 85kDa- Galnt17−/− | |||||
| HS90B_MOUSE | 3727 | 109 | 35 | 7.46 | Heat shock protein HSP 90-beta/ Hsp90ab1 |
| HS90A_MOUSE | 3031 | 90 | 28 | 3.71 | Heat shock protein HSP 90-alpha/ Hsp90aa1 |
| ACON_MOUSE | 818 | 30 | 16 | 1.32 | Aconitate hydratase/ Acon |
Output from the Mascot 2.7 analysis tool, with SwissProt matches ranked by score and cutoff of false discovery rate ≤ 0.01.
a.
_emPAI = 10PAI − 1, which is an approximation for relative protein abundance in a mixture (Ishihama et al., 2005).