Figure 4.

Hsp70 serves as a ligand of TLR4 to initiate the inflammatory cascade in hepatocytes. (A) Immunofluorescence dual labeling of TLR4 (green), Hsp70 (red), and colocalization (yellow) taken at 60× magnification, displayed with a scale of 10 μm in the liver sections from Chow, Chow + PP2A inhibitor, MASLD, MASLD + PP2A inhibitor, and NLRP3 KO MASLD + PP2A inhibitor mouse samples. Some of the colocalization events were indicated by white arrows. (B) Morphometry of Hsp70–TLR4 colocalization (yellow) events in all the groups were measured as arbitrary fluorescent units from three separate microscopic fields and plotted along the ordinate. All p-values were determined through one-way ANOVA with the significance level set at p < 0.05.