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. 2023 Nov 15;24(22):16354. doi: 10.3390/ijms242216354

Figure 5.

Figure 5

Hsp70–TLR4 ligand binding regulates the NF-κB phosphorylation to manifest hepatic inflammation. (A) Immunoblot analysis of phospho-NF-κB, total-NF-κB, IKK-β, NEMO/IKK-γ, and β-actin protein level expressions from the liver lysates of Chow, Chow + PP2A inhibitor, MASLD, MASLD + PP2A inhibitor, and NLRP3 KO MASLD + PP2A inhibitor mouse samples. (B) Densitometric analysis of phospho-NF-κB reactivity calculated as the ratio of phospho-NF-κB to total-NF-κB, displayed as mean and deviation (n = 3 in biological replicates). All p-values were determined through one-way ANOVA with the significance level set at p < 0.05. (C) Densitometric analysis of IKK-β reactivity calculated as the ratio of IKK-β to β-actin, displayed as mean and deviation (n = 3 in biological replicates). All p-values were determined through one-way ANOVA with the significance level set at p < 0.05. (D) Densitometric analysis of NEMO/IKK-γ reactivity calculated as the ratio of NEMO/IKK-γ to β-actin, displayed as mean and deviation (n = 3 in biological replicates). All p-values were determined through one-way ANOVA with the significance level set at p < 0.05.