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. 2023 Nov 15;24(22):16354. doi: 10.3390/ijms242216354

Figure 8.

Figure 8

Leptin elevates the expression of TLR4 to increase the Hsp70–TLR4 ligand binding and subsequent NF-κB phosphorylation. (A) Immunoblot analysis of TLR4, phospho-NF-κB, total-NF-κB, and β-actin protein level expressions in the H2.35 hepatocytes from Control, Hsp70, Leptin, and Leptin + Hsp70 groups. (B) Densitometric analysis of TLR4 reactivity calculated as the ratio of TLR4 to β-actin, displayed as mean and deviation (n = 3 in biological replicates). All p-values were determined through two-way ANOVA with the significance level set at p < 0.05. (C) Densitometric analysis of phospho-NF-κB reactivity calculated as the ratio of phospho-NF-κB to total-NF-κB, displayed as mean and deviation (n = 3 in biological replicates). All p-values were determined through one-way ANOVA with the significance level set at p < 0.05.