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. 2023 Nov 16;24(22):16395. doi: 10.3390/ijms242216395

Table 2.

Kinetic constants for human BChE, determined in 0.1 M phosphate buffer at pH 7.0 in the presence of Aβ1–40 at concentrations within the 0.1–10 μM range for BTC as substrate at 25 °C.

[Aβ1–40], μM Km
mM		 Kss
mM		 Vmax
μM/min		 kcat
min−1 		kcat/Km
M−1 min−1
Control 0.02 ± 0.01 2.6 ± 0.7 0.2 ± 0.02 20,909 ± 2182 1.0 ± 0.3 × 109
0.1 0.03 ± 0.01 2.0 ± 0.6 0.3 ± 0.03 22,727 ± 3000 0.8 ± 0.3 × 109
1 0.03 ± 0.01 2.3 ± 0.9 0.3 ± 0.04 24,545 ± 3636 0.9 ± 0.4 × 109
10 0.02 ± 0.01 2.0 ± 0.5 0.3 ± 0.03 25,454 ± 2727 1.5 ± 0.4 × 109