Table 2.

Summary of the different secretome preparation protocols, volumes administered, and number of administrations for the treatment of different neurological disease models.

Source	Model	Priming	Medium	Density	Conditioning Time	Concentration	T Volume Administered	N° Administrations	Ref.
rBMSC	AD	No	Serum-free RPMI	1 × 106 cells	24 h	Ultrafiltration using centrifugal filters (unspecified)	25 μL	4 or 8	[88]
hBMSC	AD	No	Neurobasal-A	2.4 × 104 cells/mL	24 h	INA	4 μL or 8 μL	1 or 2	[39]
SHED, BMSC and FibroMSC	AD	No	Serum-free DMEM	1 × 104 cells/cm2	48 h	INA	400 μL	8	[89]
hWJMSC (differentiate into Ols)	EAE	No	Serum-free DMEM/F12	INA	72 h	100-fold with ultrafiltration using 3 kDa cut-off	140 μL	14	[96]
rASC	HIE	No	BME	4 × 106 cells/cm2	24 h	250-fold by 10,000 cut-off	10 μL	1	[36]
PSC-EMSC	HIE	No	Serum-free α-MEM	9.2 × 104 cells/cm2	24 h	Removal of ions and molecules below 1kDa	84 μL	7	[112]
hDPSC/hBMSC	Hippocampal neurodegeneration	No	Serum-free basal	NA	24 h	Not concentrated or diluted	8 μL	1	[90]
hBMSC	Impaired astrocytic exocytosis (transgenic model)	No	Neurobasal A	4.0 × 103 cells/cm2	24 h	INA	0.5 μL	2	[131]
hUCMSC	NA	No	Serum-free DMEM	1 × 104 cells/cm2	72 h	10-fold by 3 kDa cut-off	200 µL	1	[21]
hUCPVC	NA	No	Neurobasal A	4.0 × 103 cells/cm2	24 h	INA	0.5 μL	1	[16]
rBMSC	Neuropathic pain	No	Serum-free DMEM	7 × 106 cells	24 h	15-fold using ultrafiltration units (unspecified)	100 μL	1	[133]
rBMSC	SCI	No	DMEM with low glucose and with/FBS	5 × 103 cells/cm2	24 h	INA	480 µL	16	[132]
hASC	Stroke	No	Serum-free α-MEM	INA	48 h	INA	0.5 μL	1	[103]
rBMSC	Stroke	No	DMEM/F12 supplemented with 2% LE rat serum	INA	24 h	10-fold by 5 kDa cut-off	INA	1	[102]
hESCMSC	Stroke	No	DMEM containing 0.05% human serum albumin and 2 mM L-glutamine, without FBS	INA	24 h	100-fold with 3 kDa cut-off	5 μL or 10 μL	1 or 2	[104]
hSHEDMSC, hBMSC	Stroke	No	Serum-free DMEM	4 × 105 cells/cm2	48 h	INA	100 μL	13	[101]
hUCMSC	Stroke	No	Serum-free DMEM/F12	1 × 104 cells/cm2	24 h	INA	140 μL	14	[100]
hBMSC	TBI	No	Serum-free DMEM	2 × 106 cells/cm2	24 h	25-fold by 3 kDa cut-off	≈275 μL	INA	[37]
hBMSC	TBI	No	Serum-free DMEM	2 × 106 cells/cm2	24 h	25-fold by 3 kDa cut-off	≈1699 μL	6	[119]
hUCMSC	TBI	No	Serum-free, low-glucose DMEM	2 × 106cells	24 h	100 kDa cut-off (unspecified)	3 μL	1	[25]
hUCPVC	TBI	No	Serum-free EBM-2	2.5 × 103 cells/cm2	72 h	NA	4 μL	1	[120]

hSHED—Human exfoliated deciduous teeth; hUCMSC—Human Umbilical Cord; hBMSC—Human Bone Marrow; hUCPVC—Human Umbilical Cord Perivascular Cells; hESCMSC—Human Embryonic stem cell-derived mesenchymal stem cells; hASC—Human Adipose Tissue; hPSC-EMSC—Human pluripotent stem cell-derived ectomesenchymal stem cells; hDPSCs—Human Dental Pulp; hWJMSC—Human Wharton’s Jelly; INA—Information not available; NA—Not applicable; DMEM—Dulbecco’s Modified Eagle Medium; EBM—Endothelial Basal Medium; RPMI—Roswell Park Memorial Institute Medium; BME—Basal Medium Eagle; HIE—Hypoxic-ischaemic Encephalopathy; TBI—traumatic brain injury; SCI—spinal cord injury; EAE—Experimental Autoimmune Encephalomyelitis; AD—Alzheimer’s disease.