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. 2023 Nov 14;24(22):16305. doi: 10.3390/ijms242216305

Figure 6.

Figure 6

Functional analysis of rose bHLH transcription factor gene RcbHLH112. (A) Compromised B. cinerea resistance upon silencing of RcbHLH112 at 60 hpi post inoculation. A recombinant tobacco rattle virus (TRV) targeting RcbHLH112 (TRV-RcbHLH112) was used for the gene silencing, and a TRV with GFP sequence (TRV-GFP) was used as the control. (B) Quantification of B. cinerea disease lesions on TRV-RcbHLH112- and TRV-GFP-inoculated rose petal discs. The graph shows the lesion size from three biological replicates (n = 48) with the standard deviation. (C) Expression of RcbHLH112 relative to that in the control at 6 days post silencing, before the infection with B. cinerea (0 hpi). All statistical analyses were performed using Student’s t-test; *** p < 0.001.