. 2023 Nov 14;24(22):16305. doi: 10.3390/ijms242216305

Table 5.

List of primers used in this study.

Gene Name	Accession Number	Primer Sequence (5′-3′)	Amplicon Length	Ta	Tm	Amplification Efficiency
RcbHLH29	RchiOBHm_Chr2g0126861	F: GGTTCCACCCTAGAGGTTGTT	110 bp	60 °C	81.69	2.005
RcbHLH29	RchiOBHm_Chr2g0126861	R: CTGCACGGACTAGGTGAAGT	110 bp	60 °C	81.69	2.005
RcbHLH60	RchiOBHm_Chr4g0445091	F: CGATGAGTTTGGACCACCGA	116 bp	60 °C	84.1	1.972
RcbHLH60	RchiOBHm_Chr4g0445091	R: CCTCAGCTTTGGCCTCAAGA	116 bp	60 °C	84.1	1.972
RcbHLH80	RchiOBHm_Chr6g0246251	F: ACACAAACCAAGTGGGGGTT	102 bp	60 °C	85.27	1.968
RcbHLH80	RchiOBHm_Chr6g0246251	R: GTTCCCTGACTGGCCTTCAA	102 bp	60 °C	85.27	1.968
RcbHLH112	RchiOBHm_Chr7g0193761	F: CGATCTTGCAGCCTCCTACA	120 bp	60 °C	82.43	2.024
RcbHLH112	RchiOBHm_Chr7g0193761	R: CAACCTTGATCCGACCACCA	120 bp	60 °C	82.43	2.024
RcUBI2	RchiOBHm_Chr1g0359561	F: GCCCTGGTGCGTTCCCAACTG	82 bp	60 °C	82.43	2.024
RcUBI2	RchiOBHm_Chr1g0359561	R: CCTGCGTGTCTGTCCGCATTG	82 bp	60 °C	82.43	2.024

Ta: amplification temperature; Tm: melting temperature.