TABLE 1.
NADP-GDH and GS activities in A. nidulans and A. oryzae wild-type and areA mutant strains
| Enzyme | Strain | Relevant genotype | Activity (U) with the following nitrogen sourcesa:
|
|||
|---|---|---|---|---|---|---|
| Glutamate | Ammonium | Ammonium (50 mM) | Glutamine | |||
| NADP-GDH | A. nidulans MH1 | Wild type | 45 (13) | 84 (9) | 48 (8) | 105 (20) |
| A. nidulans MH5699 | ΔareA::riboB | NDb | 28 (4) | 21 (3) | ND | |
| A. oryzae IFO4177 | Wild type | 100 (30) | 149 (26) | 87 (16) | 207 (32) | |
| A. oryzae TOC913 | ΔareA::pyrG | ND | 39 (8) | 18 (4) | ND | |
| GS | A. nidulans MH1 | Wild type | 370 (50) | 121 (26) | ND | 65 (11) |
| A. nidulans MH5699 | ΔareA::riboB | ND | 111 (31) | ND | ND | |
| A. oryzae IFO4177 | Wild type | 278 (8) | 88 (10) | ND | 61 (19) | |
| A. oryzae TOC913 | ΔareA::pyrG | ND | 72 (29) | ND | ND | |
a
Mycelia were grown for 16 h on glucose minimal media containing nitrogen sources at a concentration of 10 mM unless indicated otherwise. Crude cell extracts were prepared, and NADP-GDH and GS activities were determined as described in the text. Standard errors are shown in parentheses.
b
ND, not determined.