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. 2023 Nov 9;24(22):16110. doi: 10.3390/ijms242216110

Figure 3.

Figure 3

Knockout of RNF138 in mouse cells promotes virus-induced IRF3 activation. (A) The effects of RNF138 deficiency on the SeV or VSV-induced transcription of downstream genes. RNF138-KO and control RAW264.7 cells were infected with SeV or VSV for 8 h before qPCR analysis. (B) The effects of RNF138 deficiency on SeV-induced phosphorylation of IRF3 (Ser396). RNF138-deficient (KO) RAW264.7 or L929 clones were generated via the CRISPR-Cas9 method. RNF138-KO and the control RAW264.7 or L929 cells were infected with SeV for 8 h before immunoblotting analysis with the indicated antibodies. (C) The effects of RNF138 deficiency on the VSV-induced phosphorylation of IRF3 (Ser396). RNF138-KO and control RAW264.7 or L929 cells were infected with VSV for 8 h before immunoblotting analysis with the indicated antibodies. ** p < 0.01, *** p < 0.001 (unpaired t-test). Data represent at least two experiments with similar results (mean ± SD, n = 3 independent samples in (A)).